Abstract
We developed a new efficient method for the synthesis of important indoxyl glycoside substrates for β-glucosidase and β-galactosidase by using 1-acetylindol-3-ones as intermediates. This method was used to synthesise novel precipitating fluorogenic substrates for β-glucosidase based on 2-(benzothiazol-2'-yl)-phenols. We also assessed the application of these substrates in the detection of foodborne pathogenic bacteria.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacteria / enzymology
-
Bacteria / isolation & purification*
-
Chemistry Techniques, Synthetic
-
Fluorescent Dyes / chemical synthesis*
-
Fluorescent Dyes / chemistry
-
Fluorescent Dyes / metabolism*
-
Food Microbiology
-
Glycosides / chemical synthesis*
-
Glycosides / chemistry
-
Glycosides / metabolism*
-
Indoles / chemistry
-
beta-Galactosidase / metabolism*
-
beta-Glucosidase / metabolism*
Substances
-
Fluorescent Dyes
-
Glycosides
-
Indoles
-
beta-Glucosidase
-
beta-Galactosidase