Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies

Sae Hayakumo; Shinichi Arakawa; Masayoshi Takahashi; Keiko Kondo; Yoshihiro Mano; Yuichi Izumi

doi:10.1088/1468-6996/15/5/055003

Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies

Sci Technol Adv Mater. 2014 Sep 8;15(5):055003. doi: 10.1088/1468-6996/15/5/055003. eCollection 2014 Oct.

Authors

Sae Hayakumo¹, Shinichi Arakawa², Masayoshi Takahashi³, Keiko Kondo², Yoshihiro Mano⁴, Yuichi Izumi⁵

Affiliations

¹ Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
² Department of Lifetime Oral Health Care Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
³ National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.
⁴ Hyperbaric Medical Center, Hospital of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
⁵ Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan; Global Center of Excellence (GCOE) Program, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.

Abstract

The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (<10 CFUs mL^-1) after only 0.5 min of exposure. There were only minor decreases in the viability of oral tissue cells after 24 h of exposure to NBW3. These results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising.

Keywords: antiseptic; cytotoxicity; ozone nano-bubble water; periodontitis; periodontopathic bacteria.