Polymerase chain reaction (PCR) based amplification of hmcp 3 and hmcp 6 cysteine protease genes of Haemonchus contortus from small ruminants

J Parasit Dis. 2016 Dec;40(4):1313-1316. doi: 10.1007/s12639-015-0679-8. Epub 2015 Mar 21.

Abstract

Haemonchus contortus is a haematophagous nematode in small ruminants population and causes anaemia, weakness and mortality especially in young animals. In the present study, hmcp3 partial gene of 836 bp and hmcp6 full length gene of 1041 bp were amplified from the cDNA of Bareilly isolate of adult male H. contortus by polymerase chain reaction. Further gene characterization and expression studies are warranted to know the immunoprophylactic potential of hmcp3 and hmcp6 proteins of H. contortus.

Keywords: Cysteine protease; Haemonchus contortus; PCR; hmcp3; hmcp6.