Na⁺,K⁺-ATPase as the Target Enzyme for Organic and Inorganic Compounds

Vesna Vasić; Tatjana Momić; Marijana Petković; Danijela Krstić

doi:10.3390/s8128321

Na⁺,K⁺-ATPase as the Target Enzyme for Organic and Inorganic Compounds

Sensors (Basel). 2008 Dec 15;8(12):8321-8360. doi: 10.3390/s8128321.

Authors

Vesna Vasić¹, Tatjana Momić², Marijana Petković³, Danijela Krstić⁴

Affiliations

¹ Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, 11001 Belgrade, Republic of Serbia. evasic@vinca.rs.
² Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, 11001 Belgrade, Republic of Serbia. momict@vinca.rs.
³ Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, 11001 Belgrade, Republic of Serbia. marijanapetkovic@vinca.rs.
⁴ Institute of Medicinal Chemistry, University School of Medicine, University of Belgrade, Višegradska 12, Belgrade, Republic of Serbia. krsticdana@yahoo.com.

Abstract

This paper gives an overview of the literature data concerning specific and non specific inhibitors of Na⁺,K⁺-ATPase receptor. The immobilization approaches developed to improve the rather low time and temperature stability of Na⁺,K⁺-ATPase, as well to preserve the enzyme properties were overviewed. The functional immobilization of Na⁺,K⁺-ATPase receptor as the target, with preservation of the full functional protein activity and access of various substances to an optimum number of binding sites under controlled conditions in the combination with high sensitive technology for the detection of enzyme activity is the basis for application of this enzyme in medical, pharmaceutical and environmental research.

Keywords: K+-ATPase; Na+; detection; inhibition; sensor; toxic agents.

Publication types

Review