Emergence of blaKPC-3-Tn4401a in Klebsiella pneumoniae ST512 in the municipal wastewater treatment plant and in the university hospital of a town in central Italy

Mariagrazia Perilli; Carlo Bottoni; Eugenio Pontieri; Bernardetta Segatore; Giuseppe Celenza; Domenico Setacci; Pierangelo Bellio; Roberto Strom; Gianfranco Amicosante

doi:10.1016/j.jgar.2013.07.002

Emergence of bla_KPC-3-Tn4401a in Klebsiella pneumoniae ST512 in the municipal wastewater treatment plant and in the university hospital of a town in central Italy

J Glob Antimicrob Resist. 2013 Dec;1(4):217-220. doi: 10.1016/j.jgar.2013.07.002. Epub 2013 Aug 20.

Authors

Mariagrazia Perilli¹, Carlo Bottoni², Eugenio Pontieri², Bernardetta Segatore², Giuseppe Celenza², Domenico Setacci², Pierangelo Bellio², Roberto Strom³, Gianfranco Amicosante²

Affiliations

¹ Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy. Electronic address: perilli@univaq.it.
² Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
³ Dipartimento di Biotecnologie Cellulari ed Ematologia, Sapienza Università di Roma, Rome, Italy.

PMID: 27873616
DOI: 10.1016/j.jgar.2013.07.002

Abstract

In this study, 20 carbapenem-resistant environmental Klebsiella pneumoniae strains were found to correlate with 18 clinical K. pneumoniae isolates from the teaching hospital of L'Aquila city, Italy. All strains analysed by multilocus sequence typing (MLST) were included in the same clone (ST512), and pulsed-field gel electrophoresis demonstrated a genetic relationship between the clinical isolates and most environmental strains. Both environmental and clinical strains harboured the same mobile genetic elements: transposon Tn4401a including a bla_KPC-3 determinant; and a class 1 integron with the gene cassette aadA2.

Keywords: Carbapenemases; Environment; Integrons; KPC; Transposons; Wastewater.