NDM-1- and OXA-163-producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012

Mohammed O Abdelaziz; Celestino Bonura; Aurora Aleo; Teresa Fasciana; Caterina Mammina

doi:10.1016/j.jgar.2013.06.003

NDM-1- and OXA-163-producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012

J Glob Antimicrob Resist. 2013 Dec;1(4):213-215. doi: 10.1016/j.jgar.2013.06.003. Epub 2013 Aug 2.

Authors

Mohammed O Abdelaziz¹, Celestino Bonura², Aurora Aleo², Teresa Fasciana², Caterina Mammina³

Affiliations

¹ Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
² Department of Sciences for Health Promotion and Mother-Child Care 'G. D'Alessandro', University of Palermo, Via del Vespro 133, I-90127 Palermo, Italy.
³ Department of Sciences for Health Promotion and Mother-Child Care 'G. D'Alessandro', University of Palermo, Via del Vespro 133, I-90127 Palermo, Italy. Electronic address: caterina.mammina@unipa.it.

PMID: 27873615
DOI: 10.1016/j.jgar.2013.06.003

Abstract

Here we describe carbapenem resistance determinants in two Klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in Cairo, Egypt. PCR and sequencing were used to detect and characterise β-lactamase genes. Clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The first K. pneumoniae isolate carried the bla_NDM-1 gene and the second isolate carried the bla_OXA-163 gene. Both isolates co-expressed the extended-spectrum β-lactamase CTX-M-15. The two isolates belonged to different sequence types (STs), ST11 and ST16, respectively. No history of travel was established for the two patients. The first identification of NDM-1-producing K. pneumoniae in Egypt adds further evidence to the spread of NDM-1-producing Gram-negative micro-organisms in North Africa. The additional detection of bla_OXA-163 in a K. pneumoniae isolate confirms its endemic presence in a critical healthcare setting of this geographic area.

Keywords: Antimicrobial drug resistance; Carbapenemase; Klebsiella pneumoniae; NDM-1; OXA-163.