Abstract
Recent developments in mouse models that harbor part of a human immune system have proved extremely valuable to study the in vivo immune response to human specific pathogens such as Epstein-Barr virus. Over the last decades, advances in immunodeficient mouse strains that can be used as recipients for human immune cells have greatly enhanced the use of these models. Here, we describe the generation of mice with reconstituted human immune system (HIS mice) using immunocompromised mice transplanted with human CD34+ hematopoietic stem cells. We will also describe how such mice, in which human immune cells are generated de novo, can be used to study EBV infection.
Keywords:
CD34+; Epstein-Barr virus; Humanized mice; Latency.
MeSH terms
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Animals
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B-Lymphocytes / immunology
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B-Lymphocytes / metabolism
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B-Lymphocytes / virology
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Biomarkers
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Cell Communication / immunology*
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Disease Models, Animal
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Epstein-Barr Virus Infections / immunology*
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Epstein-Barr Virus Infections / metabolism
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Epstein-Barr Virus Infections / virology*
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Gene Expression Regulation, Viral
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Genes, Viral
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / immunology*
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Host-Pathogen Interactions / immunology*
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Humans
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Immune System / cytology*
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Immune System / immunology*
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Immune System / metabolism
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Immunophenotyping
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Lymph Nodes / immunology
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Lymph Nodes / metabolism
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Lymph Nodes / virology
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Mice
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Mice, Inbred NOD
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Mice, Knockout
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Spleen / immunology
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Spleen / metabolism
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Spleen / virology
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism
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T-Lymphocytes / virology
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Transplantation Chimera
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Virus Latency / immunology
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Virus Replication