A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases

Chem Commun (Camb). 2016 Dec 1;52(98):14129-14132. doi: 10.1039/c6cc07793e.

Abstract

Derivatization of a 5'-(vinylsulfonylaminodeoxy)adenosine scaffold with a clickable functionality provided an activity-based probe that was used to label native carrier protein (CP) motifs in nonribosomal peptide synthetases (NRPSs). When coupled with a fluorescent tag, this probe selectively targeted phosphopantetheinylated CPs (holo-form) from recombinant NRPS enzyme systems and in whole proteomes.

MeSH terms

  • Adenosine / analogs & derivatives
  • Adenosine / chemistry*
  • Amino Acid Motifs
  • Carrier Proteins / chemistry*
  • Carrier Proteins / metabolism
  • Molecular Probes / chemistry*
  • Molecular Structure
  • Peptide Synthases / chemistry*
  • Peptide Synthases / metabolism
  • Proteomics*

Substances

  • Carrier Proteins
  • Molecular Probes
  • Peptide Synthases
  • non-ribosomal peptide synthase
  • Adenosine