Time-resolved fluorescence spectroscopy for clinical diagnosis of actinic cheilitis

Alessandro Cosci; Marcelo Saito Nogueira; Sebastião Pratavieira; Ademar Takahama Jr; Rebeca de Souza Azevedo; Cristina Kurachi

doi:10.1364/BOE.7.004210

Time-resolved fluorescence spectroscopy for clinical diagnosis of actinic cheilitis

Biomed Opt Express. 2016 Sep 21;7(10):4210-4219. doi: 10.1364/BOE.7.004210. eCollection 2016 Oct 1.

Authors

Alessandro Cosci¹, Marcelo Saito Nogueira², Sebastião Pratavieira³, Ademar Takahama Jr⁴, Rebeca de Souza Azevedo⁴, Cristina Kurachi³

Affiliations

¹ Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi, Piazza del Viminale 1, 00184 Rome, Italy; IFAC-CNR, Istituto di Fisica Applicata "Nello Carrara," Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy; São Carlos Institute of Physics, University of São Paulo, PO Box 369, 13560-970, São Carlos, SP Brazil; a.cosci@ifac.cnr.it.
² São Carlos Institute of Physics, University of São Paulo, PO Box 369, 13560-970, São Carlos, SP Brazil; marcelosaitonogueira@gmail.com.
³ São Carlos Institute of Physics, University of São Paulo, PO Box 369, 13560-970, São Carlos, SP Brazil.
⁴ Faculdade de Odontologia de Nova Friburgo, Universidade Federal Fluminense, RJ, Brazil.

Abstract

Actinic cheilitis is a potentially malignant disorder of the lips. Its first cause is believed to be UV sun radiation. The lesion is highly heterogeneous, making the choice of area to be biopsied difficult. This study exploits the capabilities of time-resolved fluorescence spectroscopy for the identification of the most representative area to be biopsied. A preliminary study was performed on fourteen patients. A classification algorithm was used on data acquired on nine different biopsies. The algorithm discriminated between absent, mild, and moderate dysplasia with a sensitivity of 92.9%, 90.0%, and 80.0%, respectively. The false positive rate for healthy tissue (specificity) was 88.8%.

Keywords: (170.3650) Lifetime-based sensing; (170.3890) Medical optics instrumentation; (170.6510) Spectroscopy, tissue diagnostics; (170.6935) Tissue characterization; (300.6500) Spectroscopy, time-resolved.