Comparison of autohydrolysis and ionic liquid 1-butyl-3-methylimidazolium acetate pretreatment to enhance enzymatic hydrolysis of sugarcane bagasse

Muzna Hashmi; Qining Sun; Jingming Tao; Tyrone Wells Jr; Aamer Ali Shah; Nicole Labbé; Arthur J Ragauskas

doi:10.1016/j.biortech.2016.10.089

Comparison of autohydrolysis and ionic liquid 1-butyl-3-methylimidazolium acetate pretreatment to enhance enzymatic hydrolysis of sugarcane bagasse

Bioresour Technol. 2017 Jan:224:714-720. doi: 10.1016/j.biortech.2016.10.089. Epub 2016 Nov 2.

Authors

Muzna Hashmi¹, Qining Sun², Jingming Tao³, Tyrone Wells Jr², Aamer Ali Shah⁴, Nicole Labbé⁵, Arthur J Ragauskas⁶

Affiliations

¹ Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
² Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA.
³ Center of Renewable Carbon, The University of Tennessee, Knoxville, TN, USA.
⁴ Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
⁵ Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA; Center of Renewable Carbon, The University of Tennessee, Knoxville, TN, USA.
⁶ Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA; Center of Renewable Carbon, The University of Tennessee, Knoxville, TN, USA; Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: aragausk@utk.edu.

PMID: 27864135
DOI: 10.1016/j.biortech.2016.10.089

Abstract

The aim of this work was to evaluate the efficiency of an ionic liquid (IL) 1-butyl-3-methylimidazolium acetate ([C₄mim][OAc]) pretreatment (110°C for 30min) in comparison to high severity autohydrolysis pretreatment in terms of delignification, cellulose crystallinity and enzymatic digestibility. The increase in severity of autohydrolysis pretreatment had positive effect on glucan digestibility, but was limited by the crystallinity of cellulose. [C₄mim][OAc] pretreated sugarcane bagasse exhibited a substantial decrease in lignin content, reduced cellulose crystallinity, and enhanced glucan and xylan digestibility. Glucan and xylan digestibility was determined as 97.4% and 98.6% from [C₄mim][OAc] pretreated bagasse, and 62.1% and 57.5% from the bagasse autohydrolyzed at 205°C for 6min, respectively. The results indicated the improved digestibility and hydrolysis rates after [C₄mim][OAc] pretreatment when compared against a comparable autohydrolyzed biomass.

Keywords: Autohydrolysis; Hydrolysability; Ionic liquid; Sugarcane bagasse.

Publication types

Comparative Study

MeSH terms

Biomass
Biotechnology / methods*
Cellulose / chemistry*
Cellulose / metabolism
Enzymes / chemistry
Enzymes / metabolism
Glucans / chemistry
Hydrolysis
Imidazoles / chemistry*
Ionic Liquids / chemistry
Lignin / chemistry
Saccharum / chemistry*
Saccharum / metabolism
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
Xylans / chemistry

Substances

1-butyl-3-methylimidazolium
Enzymes
Glucans
Imidazoles
Ionic Liquids
Xylans
Cellulose
Lignin
bagasse