Terpene Cyclases from Social Amoebae

Angew Chem Int Ed Engl. 2016 Dec 5;55(49):15420-15423. doi: 10.1002/anie.201608971. Epub 2016 Nov 10.

Abstract

Genome sequences of social amoebae reveal the presence of terpene cyclases (TCs) in these organisms. Two TCs from Dictyostelium discoideum converted farnesyl diphosphate into (2S,3R,6S,9S)-(-)-protoillud-7-ene and (3S)-(+)-asterisca-2(9),6-diene. The enzyme mechanisms and EI-MS fragmentations of the products were studied by labeling experiments.

Keywords: biosynthesis; isotopes; mass spectrometry; social amoebae; terpenes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / chemistry
  • Alkyl and Aryl Transferases / metabolism*
  • Amoeba / enzymology*
  • Molecular Conformation
  • Terpenes / chemistry
  • Terpenes / metabolism*

Substances

  • Terpenes
  • Alkyl and Aryl Transferases