An Ex Vivo Tissue Culture Model for Anti-angiogenic Drug Testing

Mohammad S Azimi; Michelle Lacey; Debasis Mondal; Walter L Murfee

doi:10.1007/978-1-4939-3999-2_8

An Ex Vivo Tissue Culture Model for Anti-angiogenic Drug Testing

Methods Mol Biol. 2016:1464:85-95. doi: 10.1007/978-1-4939-3999-2_8.

Authors

Mohammad S Azimi¹, Michelle Lacey², Debasis Mondal³, Walter L Murfee⁴

Affiliations

¹ Department of Biomedical Engineering, Tulane University, New Orleans, LA, USA.
² Department of Mathematics, Tulane University, New Orleans, LA, USA.
³ Department of Pharmacology, Tulane University, New Orleans, LA, USA.
⁴ Department of Biomedical Engineering, Tulane University, New Orleans, LA, USA. wmurfee@tulane.edu.

PMID: 27858358
DOI: 10.1007/978-1-4939-3999-2_8

Abstract

Angiogenesis, defined as the growth of new blood vessels from existing ones, plays a key role in development, growth, and tissue repair. Its necessary role in tumor growth and metastasis has led to the creation of a new category of anti-angiogenic cancer therapies. Preclinical development and evaluation of potential drug candidates require models that mimic real microvascular networks. Here, we describe the rat mesentery culture model as a simple ex vivo assay that offers time-lapse imaging of intact microvascular network remodeling and demonstrate its application for anti-angiogenic drug testing.

Keywords: Angiogenesis; Drug screening; Endothelial cell; Mesentery; Microvascular network; Pericytes; Tissue culture.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Angiogenesis Inhibitors / pharmacology*
Animals
Drug Evaluation, Preclinical
Mesentery / blood supply
Mesentery / cytology*
Mesentery / drug effects
Microvessels / drug effects
Microvessels / ultrastructure*
Models, Biological
Rats
Rats, Wistar
Time-Lapse Imaging
Tissue Culture Techniques / methods*

Substances

Angiogenesis Inhibitors

Grants and funding

P20 GM103629/GM/NIGMS NIH HHS/United States