A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer

Tugba Ceren Gokoglan; Saniye Soylemez; Melis Kesik; Itir Bakis Dogru; Onur Turel; Recep Yuksel; Husnu Emrah Unalan; Levent Toppare

doi:10.1016/j.foodchem.2016.10.023

A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer

Food Chem. 2017 Apr 1:220:299-305. doi: 10.1016/j.foodchem.2016.10.023. Epub 2016 Oct 5.

Authors

Tugba Ceren Gokoglan¹, Saniye Soylemez², Melis Kesik³, Itir Bakis Dogru⁴, Onur Turel⁵, Recep Yuksel⁶, Husnu Emrah Unalan⁷, Levent Toppare⁸

Affiliations

¹ Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: tugba.ceren@windowslive.com.
² Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: saniyesoylemez@gmail.com.
³ Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: kesik.melis@gmail.com.
⁴ Micro and Nanotechnology, Middle East Technical University, Ankara 06800, Turkey. Electronic address: e165033@metu.edu.tr.
⁵ Department of Metallurgical and Material Engineering, Middle East Technical University, Ankara 06800, Turkey. Electronic address: e174543@metu.edu.tr.
⁶ Micro and Nanotechnology, Middle East Technical University, Ankara 06800, Turkey. Electronic address: reyuksel@metu.edu.tr.
⁷ Micro and Nanotechnology, Middle East Technical University, Ankara 06800, Turkey; Department of Metallurgical and Material Engineering, Middle East Technical University, Ankara 06800, Turkey. Electronic address: unalan@metu.edu.tr.
⁸ Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey; Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey; Department of Polymer Science and Technology, Middle East Technical University, Ankara 06800, Turkey; The Center for Solar Energy Research and Application (GUNAM), Middle East Technical University, Ankara 06800, Turkey. Electronic address: toppare@metu.edu.tr.

PMID: 27855903
DOI: 10.1016/j.foodchem.2016.10.023

Abstract

A novel flexible glucose biosensor using vertically aligned carbon nanotubes (VACNT) and a conjugated polymer (CP) was fabricated. A scaffold based on VACNT grown on aluminum foil (VACNT-Al foil) with poly (9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl)-end capped with 2,5-diphenyl-1,2,4-oxadiazole (PFLO) was used as the immobilization matrix for the glucose biosensor. Glucose oxidase (GOx) was immobilized on a modified indium tin oxide (ITO) coated polyethylene terephthalate (PET) electrode surface. The biosensor response at a potential of -0.7V versus Ag wire was followed by the decrease in oxygen level as a result of enzymatic reaction. The biosensor exhibited a linear range between 0.02mM and 0.5mM glucose and kinetic parameters (K_M^app, I_max, limit of detection (LOD) and sensitivity) were estimated as 0.193mM, 8.170μA, 7.035×10^-3mM and 65.816μA/mMcm², respectively. Scanning electron microscopy (SEM) was used for surface characterization. The constructed biosensor was applied to determine the glucose content in several beverages.

Keywords: Amperometric biosensor; Conjugated polymer; Enzyme electrode; Glucose biosensor; Glucose oxidase; Vertically aligned carbon nanotubes.

MeSH terms

Beverages / analysis
Biosensing Techniques / methods*
Enzymes, Immobilized
Glucose / analysis*
Glucose Oxidase / metabolism
Microscopy, Electron, Scanning
Nanotubes, Carbon / chemistry*
Polymers / chemistry*

Substances

Enzymes, Immobilized
Nanotubes, Carbon
Polymers
Glucose Oxidase
Glucose