One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose

Siqiang Li; Hong Wang; Juncai Ma; Guofeng Gu; Zonggang Chen; Zhongwu Guo

doi:10.1039/c6cc08366h

One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose

Chem Commun (Camb). 2016 Nov 29;52(97):13995-13998. doi: 10.1039/c6cc08366h.

Authors

Siqiang Li¹, Hong Wang¹, Juncai Ma¹, Guofeng Gu¹, Zonggang Chen¹, Zhongwu Guo²

Affiliations

¹ National Glycoengineering Research Center and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda Nan Lu, Jinan 250100, China. guofenggu@sdu.edu.cn chenzg@sdu.edu.cn.
² Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, Florida 32611, USA. zguo@chem.ufl.edu.

PMID: 27849064
DOI: 10.1039/c6cc08366h

Abstract

A new, robust one-pot four-enzyme synthetic method was developed for thymidinediphosphate-l-rhamnose starting from d-glucose-1-phosphate. The enzymes, Glc-1-P thymidylyltransferase, dTDP-Glc-4,6-dehydratase, dTDP-4-keto-6-deoxy-Glc-3,5-epimerase and dTDP-4-keto-Rha reductase were derived from Streptococcus pneumonia serotype 23F, expressed in Escherichia coli, and studied in detail to provide the first direct evidence for their functions.