Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses

Methods. 2017 Jan 15:113:132-138. doi: 10.1016/j.ymeth.2016.11.007. Epub 2016 Nov 12.

Abstract

Several recent reports have found a connection between specific aminoacyl-tRNA synthetases and the regulation of angiogenesis. As this new area of research is explored, it is important to have reliable assays to assess the specific angiogenesis functions of these enzymes. This review provides information about specific in vitro and in vivo methods that were used to assess the angiogenic functions of threonyl-tRNA synthetase including endothelial cell migration and tube assays as well as chorioallantoic membrane and tumor vascularization assays. The theory and discussion include best methods of analysis and quantification along with the advantages and limitations of each type of assay.

Keywords: Aminoacyl-tRNA synthetase; Angiogenesis methods; CAM assay; Endothelial cells; ImageJ; MetaMorph.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biological Assay*
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism
  • Cell Movement / drug effects
  • Chick Embryo
  • Chorioallantoic Membrane / blood supply
  • Chorioallantoic Membrane / drug effects*
  • Chorioallantoic Membrane / enzymology
  • Collagen / chemistry
  • Drug Combinations
  • Enzyme Inhibitors / pharmacology*
  • Female
  • Gene Expression Regulation
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Laminin / chemistry
  • Mice
  • Neovascularization, Pathologic / drug therapy*
  • Neovascularization, Pathologic / enzymology
  • Neovascularization, Pathologic / genetics
  • Neovascularization, Pathologic / pathology
  • Neovascularization, Physiologic / drug effects
  • Neovascularization, Physiologic / genetics
  • Ovarian Neoplasms / blood supply
  • Ovarian Neoplasms / drug therapy*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / pathology
  • Platelet Endothelial Cell Adhesion Molecule-1 / genetics
  • Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
  • Proteoglycans / chemistry
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • RNA, Transfer, Thr / genetics
  • RNA, Transfer, Thr / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Threonine-tRNA Ligase / antagonists & inhibitors*
  • Threonine-tRNA Ligase / genetics
  • Threonine-tRNA Ligase / metabolism
  • Transfer RNA Aminoacylation*
  • Xenograft Model Antitumor Assays

Substances

  • Biomarkers, Tumor
  • Drug Combinations
  • Enzyme Inhibitors
  • Laminin
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Proteoglycans
  • RNA, Small Interfering
  • RNA, Transfer, Thr
  • Recombinant Fusion Proteins
  • matrigel
  • Collagen
  • Threonine-tRNA Ligase