Characterization of hemicelluloses from Neolamarckia cadamba (Rubiaceae) during xylogenesis

Xianhai Zhao; Tingting Tong; HuiLing Li; Haojun Lu; Junli Ren; Aiping Zhang; Xiaomei Deng; Xiaoyang Chen; Ai-Min Wu

doi:10.1016/j.carbpol.2016.09.041

Characterization of hemicelluloses from Neolamarckia cadamba (Rubiaceae) during xylogenesis

Carbohydr Polym. 2017 Jan 20:156:333-339. doi: 10.1016/j.carbpol.2016.09.041. Epub 2016 Sep 16.

Authors

Xianhai Zhao¹, Tingting Tong¹, HuiLing Li², Haojun Lu², Junli Ren³, Aiping Zhang⁴, Xiaomei Deng², Xiaoyang Chen⁵, Ai-Min Wu⁶

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
² Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
³ State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, China.
⁴ College of Materials and Energy, South China Agricultural University, Guangzhou, China.
⁵ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
⁶ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China. Electronic address: wuaimin@scau.edu.cn.

PMID: 27842830
DOI: 10.1016/j.carbpol.2016.09.041

Abstract

In this study, hemicelluloses were isolated from the apical, middle and basal segments of N. cadamba using a 2N KOH extraction procedure. Chemical composition and structural characterization of the three hemicellulosic fractions obtained were comparatively investigated by a combination of HPLC, GPC, FTIR, ¹H,¹³C, HSQC NMR and TGA techniques. According to the sugar analysis and spectral results, (4-O-methyl) glucuronoxylan was the primary hemicellulose identified in the samples with trace levels of mannan also present. All of the three samples showed spherical polymers in 0.005M sodium phosphate solution (containing 0.02N NaCl). Xylan in the middle and basal segment stems had higher molecular weights. Our findings show that during xylogenesis in N. cadamba, changes are observed in xylan content and molecular weight.

Keywords: (4-O-methyl) glucuronoxylan; Hemicellulose; N. cadamba; Structural characterization.

MeSH terms

Magnetic Resonance Spectroscopy
Molecular Weight
Polysaccharides / chemistry*
Rubiaceae / chemistry*
Xylans / biosynthesis
Xylans / chemistry*
Xylans / isolation & purification

Substances

Polysaccharides
Xylans
hemicellulose