Analysis of Type III Secretion System Secreted Proteins

Danielle L Jessen Condry; Matthew L Nilles

doi:10.1007/978-1-4939-6649-3_8

Analysis of Type III Secretion System Secreted Proteins

Methods Mol Biol. 2017:1531:93-99. doi: 10.1007/978-1-4939-6649-3_8.

Authors

Danielle L Jessen Condry¹, Matthew L Nilles²

Affiliations

¹ Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA. danielle.condry@med.und.edu.
² Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA.

PMID: 27837484
DOI: 10.1007/978-1-4939-6649-3_8

Abstract

Secreted proteins of the T3SS vary from genus to genus. How secretion is induced in vitro also depends on the genus of bacteria. However, once those proteins are isolated the method for analyzing those proteins is largely the same. The following chapter outlines the specific induction of Yersinia secreted proteins and uniform analysis of those secreted proteins.

Keywords: Outer proteins; Secretion; Type III secretion system.

MeSH terms

Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Blotting, Western
Protein Transport
Type III Secretion Systems / genetics
Type III Secretion Systems / metabolism*
Yersinia / genetics
Yersinia / metabolism

Substances

Bacterial Outer Membrane Proteins
Bacterial Proteins
Type III Secretion Systems