Sequential anti-idiotypes define reciprocal idiotopes on the same anti-DNA antibody

Clin Immunol Immunopathol. 1989 Jan;50(1 Pt 2):S106-16. doi: 10.1016/0090-1229(89)90117-7.

Abstract

Sequential anti-idiotypes have defined a pair of idiotypes with an idiotype-anti-idiotype relationship to each other in the variable region of the monoclonal anti-DNA antibody 16/6. A rabbit immunized with 16/6 made an antibody-1 (anti-Id-16/6R) and an antibody-2 (anti-anti-Id-16/6 or anti-anti-Id 16/6M). The latter bound to M-2, a monoclonal mouse anti-Id-16/6. M-2 was used to immunize a second mouse, which produced M-16. The variable region of M-16 mimics an antigenic surface on Id-16/6 and binds to a peptide contained within the first hypervariable region of the light chain of 16/6. The complementary idiotypes of 16/6 represent a reciprotope, an idiotype/anti-idiotype pair within the variable region of the same immunoglobulin molecule which may have functional importance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / immunology
  • Antibodies, Antinuclear / immunology*
  • Antibodies, Monoclonal / immunology
  • Antigen-Antibody Reactions
  • Humans
  • Immunoglobulin Idiotypes / immunology*
  • Immunoglobulin Variable Region / immunology
  • Lupus Erythematosus, Systemic / immunology
  • Mice
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology
  • Protein Binding
  • Protein Conformation
  • Rabbits
  • Species Specificity

Substances

  • Antibodies, Anti-Idiotypic
  • Antibodies, Antinuclear
  • Antibodies, Monoclonal
  • Immunoglobulin Idiotypes
  • Immunoglobulin Variable Region
  • Peptide Fragments