Isolation of Hepatocytes and Stellate Cells from a Single Piece of Human Liver

Methods Mol Biol. 2017:1506:247-258. doi: 10.1007/978-1-4939-6506-9_17.

Abstract

Co-transplantation of hepatocytes and hepatic stellate cells has been shown to increase the engraftment of transplanted hepatocytes in the liver. Here, we describe a method for the simultaneous isolation of human primary hepatocytes and hepatic stellate cells from the same donor for co-transplantation or for use in in vitro cell culture models.

Keywords: Collagenase; Hepatic stellate cells; Hepatocyte isolation; Hepatocytes; Human; Human hepatocytes; Liver; Liver cell isolation; Perfusion; Stellate cell isolation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Separation / instrumentation
  • Cell Separation / methods*
  • Cell Survival
  • Cell Transplantation / methods
  • Cells, Cultured
  • Coculture Techniques / methods
  • Cryopreservation
  • Hepatic Stellate Cells / physiology*
  • Hepatic Stellate Cells / transplantation
  • Hepatocytes / physiology*
  • Hepatocytes / transplantation
  • Humans
  • Liver / cytology
  • Liver / surgery*
  • Perfusion / instrumentation
  • Perfusion / methods*
  • Primary Cell Culture / methods
  • Tissue Donors