Experimental Hepatocyte Transplantation in Pigs

Eiji Kobayashi; Shin Enosawa; Hiroshi Nagashima

doi:10.1007/978-1-4939-6506-9_10

Experimental Hepatocyte Transplantation in Pigs

Methods Mol Biol. 2017:1506:149-160. doi: 10.1007/978-1-4939-6506-9_10.

Authors

Eiji Kobayashi¹, Shin Enosawa², Hiroshi Nagashima³

Affiliations

¹ Department of Organ Fabrication, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
² Division for Advanced Medical Sciences, National Center for Child Health and Development, 2-10-1 Ookura, Setagaya-ku, Tokyo, 157-8535, Japan. enosawa-s@ncchd.go.jp.
³ Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, 214-8571, Japan.

PMID: 27830551
DOI: 10.1007/978-1-4939-6506-9_10

Abstract

Technical issues of experimental hepatocyte transplantation in pigs, i.e., selection of animals, anesthesia, route of transplantation, and segment-specific transplantation have described.

Keywords: Animal model; Intraportal injection; Pig; Segment-specific transplantation; Umbilical vein.

MeSH terms

Animals
Animals, Genetically Modified
Cell Transplantation / adverse effects
Cell Transplantation / instrumentation
Cell Transplantation / methods*
Female
Hepatocytes / transplantation*
Liver / anatomy & histology
Liver / surgery*
Male
Models, Animal
Portal Vein / surgery
Postoperative Care / methods
Swine
Swine, Miniature / physiology*
Tissue and Organ Harvesting / instrumentation
Tissue and Organ Harvesting / methods
Umbilical Veins / surgery