Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12

Acta Crystallogr F Struct Biol Commun. 2016 Nov 1;72(Pt 11):831-839. doi: 10.1107/S2053230X16015612. Epub 2016 Oct 27.

Abstract

Fuculose-1-phosphate aldolase (FucA) catalyses the reversible cleavage of L-fuculose 1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde. This enzyme from mesophiles and thermophiles has been extensively studied; however, there is no report on this enzyme from a psychrophile. In this study, the gene encoding FucA from Glaciozyma antarctica PI12 (GaFucA) was cloned and the enzyme was overexpressed in Escherichia coli, purified and crystallized. The tetrameric structure of GaFucA was determined to 1.34 Å resolution. The overall architecture of GaFucA and its catalytically essential histidine triad are highly conserved among other fuculose aldolases. Comparisons of structural features between GaFucA and its mesophilic and thermophilic homologues revealed that the enzyme has typical psychrophilic attributes, indicated by the presence of a high number of nonpolar residues at the surface and a lower number of arginine residues.

Keywords: Glaciozyma antarctica; crystallization; fuculose aldolase; imino cyclitols; l-fuculose metabolism; metalloenzymes; psychrophiles.

MeSH terms

  • Aldehyde-Lyases / chemistry*
  • Aldehyde-Lyases / genetics
  • Aldehyde-Lyases / metabolism
  • Amino Acid Sequence
  • Antarctic Regions
  • Binding Sites
  • Catalytic Domain
  • Cloning, Molecular
  • Crystallography, X-Ray
  • Dihydroxyacetone Phosphate / chemistry*
  • Dihydroxyacetone Phosphate / metabolism
  • Enzyme Stability
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Fungal Proteins / chemistry*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression
  • Hexosephosphates / chemistry*
  • Hexosephosphates / metabolism
  • Models, Molecular
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Protein Binding
  • Protein Conformation, alpha-Helical
  • Protein Conformation, beta-Strand
  • Protein Interaction Domains and Motifs
  • Protein Multimerization
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomycetales / chemistry*
  • Saccharomycetales / enzymology
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Fungal Proteins
  • Hexosephosphates
  • Recombinant Proteins
  • fuculose 1-phosphate
  • Dihydroxyacetone Phosphate
  • Aldehyde-Lyases
  • L-fuculosephosphate aldolase