Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India

Sasmita Panda; Smrutiti Jena; Savitri Sharma; Benu Dhawan; Gopal Nath; Durg Vijai Singh

doi:10.1371/journal.pone.0166193

Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India

PLoS One. 2016 Nov 8;11(11):e0166193. doi: 10.1371/journal.pone.0166193. eCollection 2016.

Authors

Sasmita Panda¹, Smrutiti Jena¹, Savitri Sharma², Benu Dhawan³, Gopal Nath⁴, Durg Vijai Singh¹

Affiliations

¹ Infectious Disease Biology, Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India.
² Jhaveri Microbiology Centre, LV Prasad Eye Institute, Kallam Anji Reddy Campus, LV Prasad Marg, Banjara Hills, Hyderabad-500034, India.
³ Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India.
⁴ Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India.

Abstract

The aim of this study was to determine sequence types of 34 S. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in India by MLST. The MEGA5.2 software was used to align and compare the nucleotide sequences. The advanced cluster analysis was performed to define the clonal complexes. MLST analysis showed 24 new sequence types (ST) among S. haemolyticus isolates, irrespective of sources and place of isolation. The finding of this study allowed to set up an MLST database on the PubMLST.org website using BIGSdb software and made available at http://pubmlst.org/shaemolyticus/. The data of this study thus suggest that MLST can be used to study population structure and diversity among S. haemolyticus isolates.

MeSH terms

Bacterial Typing Techniques / methods
Cluster Analysis
DNA, Bacterial / genetics
Genetic Variation / genetics
Genotype
Humans
India
Multilocus Sequence Typing / methods
Phylogeny
Sequence Analysis, DNA / methods
Staphylococcal Infections / microbiology*
Staphylococcus haemolyticus / genetics*

Substances

DNA, Bacterial

Grants and funding

DVS was supported by a grant from Science and Engineering Research Board, New Delhi (Grant no. SB/SO/HS-0203/2012). SP and SJ were supported by Senior Research Fellowships from Indian Council of Medical Research, New Delhi and Department of Science and Technology, New Delhi. The funder had no role in study design, data collection, and analysis, decision to publish or preparation of the manuscript.