Abstract
The influence of GABA receptor agonists on the terminal differentiation in vitro of dopaminergic (DA) neurons derived from IPS cells was investigated. GABA-A agonist muscimol induced transient elevation of intracellular Ca2+ level ([Ca2+] i ) in the investigated cells at days 5 to 21 of differentiation. Differentiation of cells in the presence of muscimol reduced tyrosine hydroxylase expression. Thus, the presence of active GABA-A receptors, associated with phenotype determination via Ca2+-signalling was demonstrated in differentiating human DA neurons.
MeSH terms
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Baclofen / administration & dosage
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Calcium / metabolism
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Calcium Signaling / drug effects
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Calcium Signaling / physiology
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Cell Differentiation / drug effects
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Cell Differentiation / physiology
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Cells, Cultured
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Dopaminergic Neurons / cytology*
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Dopaminergic Neurons / drug effects
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Dopaminergic Neurons / metabolism*
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GABA Agonists / administration & dosage*
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Humans
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / drug effects
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Induced Pluripotent Stem Cells / metabolism*
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Muscimol / administration & dosage
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Receptors, GABA-A / metabolism*
Substances
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GABA Agonists
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Receptors, GABA-A
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Muscimol
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Baclofen
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Calcium