Reprogramming Enhancers in Somatic Cell Nuclear Transfer, iPSC Technology, and Direct Conversion

Stem Cell Rev Rep. 2017 Feb;13(1):24-34. doi: 10.1007/s12015-016-9697-x.

Abstract

Direct conversion of cell fate is a core technology for future regenerative medicine. However, the low reprogramming efficiency and low reproducibility are the largest obstacles blocking translational research of direct conversion technology. This review lists the major reprogramming enhancers verified in the representative reprogramming technologies of somatic cell nuclear transfer, induced pluripotent stem cell technology, and direct conversion, and categorizes them according to their functions. Ultimately, we strive to provide a practical plan to overcome the low reprogramming efficiency and low reproducibility of direct conversion.

Keywords: Direct conversion; Induced pluripotent stem cells; Reprogramming; Reprogramming efficiency; Reprogramming enhancer; Somatic cell nuclear transfer.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods
  • Cell Differentiation*
  • Cellular Reprogramming*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Nuclear Transfer Techniques*
  • Regenerative Medicine / methods
  • Regenerative Medicine / trends
  • Reproducibility of Results