Abstract
The expression of several genes which functions are associated with cellular senescence was analyzed in multipotent mesenchymal stromal cells during long-term cultivation at different oxygen levels (20, 5, and 1%) using the RT² Profiler™ PCR Array Human Cellular Senescence system (Qiagen, United States). It was established that replicative senescence processes develop most actively in the cells cultured under the standard conditions (20% O2). The most significant changes were observed in the expression of CCND1, ID1, IGF1, PIK3CA, and SERPINE1 genes.
MeSH terms
-
Adipose Tissue / metabolism
-
Cell Hypoxia / physiology*
-
Cells, Cultured
-
Cellular Senescence / physiology*
-
Class I Phosphatidylinositol 3-Kinases
-
Cyclin D1 / metabolism
-
Early Growth Response Protein 1 / metabolism
-
Eye Proteins / metabolism
-
Gene Expression
-
Glycogen Synthase Kinase 3 beta / metabolism
-
Humans
-
Inhibitor of Differentiation Protein 1 / metabolism
-
Insulin-Like Growth Factor I / metabolism
-
Mesenchymal Stem Cells / metabolism*
-
Nerve Growth Factors / metabolism
-
Phosphatidylinositol 3-Kinases / metabolism
-
Polymerase Chain Reaction
-
Proto-Oncogene Proteins c-mdm2 / metabolism
-
RNA, Messenger / metabolism
-
Serpins / metabolism
Substances
-
CCND1 protein, human
-
EGR1 protein, human
-
Early Growth Response Protein 1
-
Eye Proteins
-
ID1 protein, human
-
IGF1 protein, human
-
Inhibitor of Differentiation Protein 1
-
Nerve Growth Factors
-
RNA, Messenger
-
Serpins
-
pigment epithelium-derived factor
-
Cyclin D1
-
Insulin-Like Growth Factor I
-
MDM2 protein, human
-
Proto-Oncogene Proteins c-mdm2
-
Phosphatidylinositol 3-Kinases
-
Class I Phosphatidylinositol 3-Kinases
-
PIK3CA protein, human
-
GSK3B protein, human
-
Glycogen Synthase Kinase 3 beta