Bacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units

Kyle E Novakowski; Dessi Loukov; Vikash Chawla; Dawn M E Bowdish

doi:10.1007/978-1-4939-6581-6_20

Bacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units

Methods Mol Biol. 2017:1519:297-309. doi: 10.1007/978-1-4939-6581-6_20.

Authors

Kyle E Novakowski^{1

2

3}, Dessi Loukov^{1

2

3}, Vikash Chawla^{1

2

3}, Dawn M E Bowdish^{4

5

6}

Affiliations

¹ Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada, L8N 3Z5.
² McMaster Immunology Research Centre, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.
³ M. G. DeGroote Institute for Infectious Disease Research, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.
⁴ Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada, L8N 3Z5. bowdish@mcmaster.ca.
⁵ McMaster Immunology Research Centre, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1. bowdish@mcmaster.ca.
⁶ M. G. DeGroote Institute for Infectious Disease Research, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1. bowdish@mcmaster.ca.

PMID: 27815888
DOI: 10.1007/978-1-4939-6581-6_20

Abstract

Herein we provide a colony forming unit (CFU)-based counting method for quantitating the bacterial binding, phagocytosis, and killing capacity of phagocytes. Although these functions can be measured by immunofluorescence and dye-based assays, quantitating CFUs is comparatively inexpensive and easy to perform. The protocol described below is easily modified for use with different phagocytes (e.g., macrophages, neutrophils, cell lines), types of bacteria or opsonic conditions.

Keywords: Bacterial killing; Gentamicin protection; Macrophage; Particle binding; Phagocyte; Phagocytosis.

MeSH terms

Animals
Bacteria / drug effects
Bacteria / metabolism*
Colony-Forming Units Assay / methods*
Gentamicins / pharmacology
Macrophages / drug effects
Macrophages / metabolism
Microbial Viability* / drug effects
Phagocytosis* / drug effects
Sheep

Substances

Gentamicins