Induction of Both Local Immune Response in Mice and Protection in a Rabbit Model by Intranasal Immunization with Modified Vaccinia Ankara Virus Expressing a Secreted Form of Bovine Herpesvirus 1 Glycoprotein D

Viral Immunol. 2017 Jan/Feb;30(1):70-76. doi: 10.1089/vim.2016.0090. Epub 2016 Nov 3.

Abstract

In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.

Keywords: vaccines; vectors; veterinary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antibodies, Viral / blood
  • Disease Models, Animal
  • Drug Carriers*
  • Female
  • Herpesviridae Infections / immunology
  • Herpesviridae Infections / prevention & control*
  • Herpesvirus 1, Bovine / immunology*
  • Herpesvirus Vaccines / administration & dosage
  • Herpesvirus Vaccines / genetics
  • Herpesvirus Vaccines / immunology*
  • Mice, Inbred BALB C
  • Rabbits
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • Vaccinia virus / genetics*
  • Viral Proteins / genetics
  • Viral Proteins / immunology*

Substances

  • Antibodies, Viral
  • Drug Carriers
  • Herpesvirus Vaccines
  • Vaccines, Synthetic
  • Viral Proteins
  • bovine herpesvirus type-1 glycoproteins