Making better CRISPR libraries

Elife. 2016 Nov 3:5:e21863. doi: 10.7554/eLife.21863.

Shiyou Zhu^{1

2}, Wensheng Wei^{1

3

4}

¹ Biodynamic Optical Imaging Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.
² Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, Peking University, Beijing, China.
³ Beijing Advanced Innovation Center for Genomics, Peking University, Beijing, China.
⁴ Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.

Abstract

A new algorithm improves the performance of CRISPR-based genetic screens in mammals.

Keywords: CRISPR; chromosomes; computational biology; genes; genetic screening; human; nucleosomes; systems biology.