Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites

Guojuan Zhang; Zhiguang Liu; Li Wang; Yujing Guo

doi:10.3390/s16111803

Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites

Sensors (Basel). 2016 Oct 28;16(11):1803. doi: 10.3390/s16111803.

Authors

Guojuan Zhang^{1

2}, Zhiguang Liu³, Li Wang⁴, Yujing Guo⁵

Affiliations

¹ Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. 201422901011@email.sxu.edu.cn.
² College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. 201422901011@email.sxu.edu.cn.
³ Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. liuzg@sxu.edu.cn.
⁴ Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. wangli@sxu.edu.cn.
⁵ Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. guoyj@sxu.edu.cn.

Abstract

In this work, a novel electrochemical aptasensor was developed for sensitive and selective detection of myoglobin based on meso-tetra (4-carboxyphenyl) porphyrin-functionalized graphene-conjugated gold nanoparticles (TCPP-Gr/AuNPs). Due to its good electric conductivity, large specific surface area, and excellent mechanical properties, TCPP-Gr/AuNPs can act as an enhanced material for the electrochemical detection of myoglobin. Meanwhile, it provides an effective matrix for immobilizing myoglobin-binding aptamer (MbBA). The electrochemical aptasensor has a sensitive response to myoglobin in a linear range from 2.0 × 10^-11 M to 7.7 × 10^-7 M with a detection limit of 6.7 × 10^-12 M (S/N = 3). Furthermore, the method has the merits of high sensitivity, low price, and high specificity. Our work will supply new horizons for the diagnostic applications of graphene-based materials in biomedicine and biosensors.

Keywords: AuNPs; TCPP; graphene; myoglobin.