Morphological Effect of the New Antifungal Agent ME1111 on Hyphal Growth of Trichophyton mentagrophytes, Determined by Scanning and Transmission Electron Microscopy

Yayoi Nishiyama; Sho Takahata; Shigeru Abe

doi:10.1128/AAC.01195-16

Morphological Effect of the New Antifungal Agent ME1111 on Hyphal Growth of Trichophyton mentagrophytes, Determined by Scanning and Transmission Electron Microscopy

Antimicrob Agents Chemother. 2016 Dec 27;61(1):e01195-16. doi: 10.1128/AAC.01195-16. Print 2017 Jan.

Authors

Yayoi Nishiyama¹, Sho Takahata², Shigeru Abe³

Affiliations

¹ Teikyo University Institute of Medical Mycology, Tokyo, Japan ynishiya@main.teikyo-u.ac.jp.
² Meiji Seika Pharma Co., Ltd., Tokyo, Japan.
³ Teikyo University Institute of Medical Mycology, Tokyo, Japan.

Abstract

The effects of ME1111, a novel antifungal agent, on the hyphal morphology and ultrastructure of Trichophyton mentagrophytes were investigated by using scanning and transmission electron microscopy. Structural changes, such as pit formation and/or depression of the cell surface, and degeneration of intracellular organelles and plasmolysis were observed after treatment with ME1111. Our results suggest that the inhibition of energy production by ME1111 affects the integrity and function of cellular membranes, leading to fungal cell death.

Keywords: antifungal agents; electron microscopy; mode of action; morphology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antifungal Agents / pharmacology*
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Trichophyton / drug effects*
Trichophyton / ultrastructure

Substances

Antifungal Agents