A highly sensitive and selective electrochemical determination of non-steroidal prostate anti-cancer drug nilutamide based on f-MWCNT in tablet and human blood serum sample

R Karthik; R Sasikumar; Shen-Ming Chen; J Vinoth Kumar; A Elangovan; V Muthuraj; P Muthukrishnan; Fahad M A Al-Hemaid; M Ajmal Ali; Mohamed S Elshikh

doi:10.1016/j.jcis.2016.10.047

A highly sensitive and selective electrochemical determination of non-steroidal prostate anti-cancer drug nilutamide based on f-MWCNT in tablet and human blood serum sample

J Colloid Interface Sci. 2017 Feb 1:487:289-296. doi: 10.1016/j.jcis.2016.10.047. Epub 2016 Oct 19.

Authors

Affiliations

¹ Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC.
² Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: smchen78@ms15.hinet.net.
³ Department of Chemistry, VHNSN College, Virudhunagar 626001, Tamil Nadu, India.
⁴ Department of Chemistry, Thiagarajar College, Madurai 625009, Tamil Nadu, India.
⁵ Department of Chemistry, Faculty of Engineering, Karpagam University, Coimbatore 641021, India.
⁶ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

PMID: 27792936
DOI: 10.1016/j.jcis.2016.10.047

Abstract

A novel electrochemical sensor based on the functionalized multiwalled carbon nanotube (f-MWCNT) was successfully developed for the sensitive and selective determination of non-steroidal prostate anti-cancer drug nilutamide in tablet and blood serum samples. The f-MWCNT was prepared by the simple reflux method and characterized by the scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Raman spectroscopy, X-ray powder diffraction (XRD) and fourier transform infrared spectroscopy (FT-IR). Interestingly, the f-MWCNT was exhibited a superior electrocatalytic activity towards the anti-cancer drug nilutamide when compared with pristine MWCNT and unmodified electrodes. Besides, the electrochemical sensor was revealed an excellent current response for the determination of nilutamide with wide linear ranges (0.01-21μM and 28-535μM), high sensitivity (11.023 and 1.412μA μM^-1cm²) and very low detection limit (LOD) 0.2nM. The developed electrochemical sensor was showed an excellent selectivity even in the presence of electrochemically active biological substances and nitro aromatic compounds. Moreover, it manifested a good reproducibility and stability. In addition, the f-MWCNT modified glassy carbon electrode (GCE) sensor was successfully applied for the detection of nilutamide in tablet and blood serum sample.

Keywords: Electrochemical sensor; Nilutamide; Serum sample; Tablet; f-MWCNT.

MeSH terms

Androgen Antagonists / blood*
Antineoplastic Agents / blood*
Biosensing Techniques*
Electrochemical Techniques*
Electrodes
Humans
Imidazolidines / blood*
Limit of Detection
Nanotubes, Carbon / chemistry*
Nanotubes, Carbon / ultrastructure
Reproducibility of Results
Tablets

Substances

Androgen Antagonists
Antineoplastic Agents
Imidazolidines
Nanotubes, Carbon
Tablets
nilutamide