Dataset reporting detection of breast cancer-related HER2I655V polymorphism using allele-specific polymerase chain reaction

Bugi Ratno Budiarto; Desriani

doi:10.1016/j.dib.2016.09.033

Dataset reporting detection of breast cancer-related HER2^I655V polymorphism using allele-specific polymerase chain reaction

Data Brief. 2016 Sep 24:9:689-695. doi: 10.1016/j.dib.2016.09.033. eCollection 2016 Dec.

Authors

Bugi Ratno Budiarto¹, Desriani¹

Affiliation

¹ Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia.

Abstract

The dataset presented in this article is related to the research article entitled "Detection of HER2 Gene Polymorphism in Breast Cancer: PCR Optimization Study" (B.R. Budiarto, Desriani, 2016) [1] with some modification in primers used and in PCR optimization strategy to eliminate false-positive result that may occur in HER2^I655V polymorphism detection. Combining a new set of primers with PCR gradient, The allele-specific PCR well performs to detect all type of breast cancer-originated HER2^I655V genotypes. The validation of this method was done using Sanger DNA sequencing, offering an alternative tool for HER2^I655V polymorphism detection in another type of cancer.

Keywords: Allele-specific PCR; Breast cancer; HER2; Ile655Val; Single nucleotide polymorphism.