Strategies for T Helper Cell Subset Differentiation from Naïve Precursors

Francesco Annunziato; Laura Maggi

doi:10.1007/978-1-4939-6548-9_11

Strategies for T Helper Cell Subset Differentiation from Naïve Precursors

Methods Mol Biol. 2017:1514:127-137. doi: 10.1007/978-1-4939-6548-9_11.

Authors

Francesco Annunziato¹, Laura Maggi²

Affiliations

¹ Department of Experimental and Clinical Medicine, University of Florence and Regenerative Medicine Unit, Careggi University Hospital Florence, Viale Pieraccini 6, Florence, 50134, Italy. francesco.annunziato@unifi.it.
² Department of Experimental and Clinical Medicine, University of Florence and Regenerative Medicine Unit, Careggi University Hospital Florence, Viale Pieraccini 6, Florence, 50134, Italy.

PMID: 27787798
DOI: 10.1007/978-1-4939-6548-9_11

Abstract

In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specific polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells.

Keywords: CD4+ naïve T cells; CD4+CD161+ T cells; Th1; Th17; Th2.

MeSH terms

CD4-Positive T-Lymphocytes / immunology*
Cell Differentiation / immunology*
Cytokines / immunology
Humans
Lymphocyte Activation / immunology
Molecular Biology / methods*
T-Lymphocyte Subsets / immunology
T-Lymphocytes, Helper-Inducer / immunology*
Th1 Cells / immunology
Th17 Cells / immunology
Th2 Cells / immunology

Substances

Cytokines