Strain parameters of Salmonellas and Lactobacteria affecting their antagonistic activity were studied using an original method. Real-time PCR was applied for measuring the decrease in the concentration of field isolates of Salmonellas isolated from chickens in the presence of field isolates of Lactobacteria also isolated from the gastrointestinal tract of chickens and broiler chickens. Dispersion analysis showed that the probability of suppression of Salmonella growth by Lactobacteria is determined by strain parameters of both Lactobacteria and Salmonellas (p<0.001). However, strain parameters of Lactobacteria were found to be more important that Salmonella strain parameters during co-culturing.
Keywords: Lactobacteria; Salmonellas; antagonistic activity; gastrointestinal microbiota; real-time polymerase chain reaction.