SERCA2a was serotonylated and may regulate sino-atrial node pacemaker activity

Biochem Biophys Res Commun. 2016 Nov 18;480(3):492-497. doi: 10.1016/j.bbrc.2016.10.082. Epub 2016 Oct 22.

Abstract

The modulation of sino-atrial node (SAN) automaticity is an essential mechanism of heart rate generation that is still not completely understood. Recent studies highlighted the importance of protein serotonylation by intracellular 5-HT during varies physiological actions. Nevertheless, the functional role of protein serotonylation in controlling SAN automaticity is largely unexplored. In this study, we screened the cardiomyocytes proteins and found that sarco(endo)plasmic reticulum Ca ATPase type 2a (SERCA2a) can be serotonylated. Simulation studies using mathematical SAN cell model showed that variation of Ca2+ affinity of SERCA2a pump cause either tachycardia or bradycardia.

Keywords: Pacemaker activity; Sarco(endo)plasmic reticulum Ca ATPase; Serotonylation; Sino-atrial node.

MeSH terms

  • Animals
  • Arrhythmias, Cardiac / physiopathology*
  • Biological Clocks*
  • Calcium Signaling*
  • Computer Simulation
  • Ion Channel Gating
  • Male
  • Mice
  • Models, Cardiovascular
  • Rats
  • Rats, Sprague-Dawley
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism*
  • Serotonin / metabolism*
  • Sinoatrial Node / physiopathology*

Substances

  • Serotonin
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases