Determination of the distributions of degrees of acetylation of chitosan

Joel Jerushan Thevarajah; Matthew Paul Van Leeuwen; Herve Cottet; Patrice Castignolles; Marianne Gaborieau

doi:10.1016/j.ijbiomac.2016.10.056

Determination of the distributions of degrees of acetylation of chitosan

Int J Biol Macromol. 2017 Feb:95:40-48. doi: 10.1016/j.ijbiomac.2016.10.056. Epub 2016 Oct 19.

Authors

Joel Jerushan Thevarajah¹, Matthew Paul Van Leeuwen², Herve Cottet³, Patrice Castignolles⁴, Marianne Gaborieau⁵

Affiliations

¹ Western Sydney University, Molecular Medicine Research Group (MMRG), Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia; Western Sydney University, Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: joel.thevarajah@westernsydney.edu.au.
² Western Sydney University, Molecular Medicine Research Group (MMRG), Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia; Western Sydney University, School of Medicine, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: m.vanleeuwen@westernsydney.edu.au.
³ Institut des Biomolécules Max Mousseron IBMM, UMR 5247, CNRS, Universiét de Montpellier, Ecole Nationale Supérieure de Chimie de Montpellier, Place Eugène Bataillon, CC 1706, 34095 Montpellier Cedex 5, France. Electronic address: herve.cottet@umontpellier.fr.
⁴ Western Sydney University, Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: p.castignolles@westernsydney.edu.au.
⁵ Western Sydney University, Molecular Medicine Research Group (MMRG), Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia; Western Sydney University, Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: m.gaborieau@westernsydney.edu.au.

PMID: 27771414
DOI: 10.1016/j.ijbiomac.2016.10.056

Abstract

Chitosan is often characterized by its average degree of acetylation. To increase chitosan's use in various industries, a more thorough characterization is necessary as the acetylation of chitosan affects properties such as dissolution and mechanical properties of chitosan films. Despite the poor solubility of chitosan, free solution capillary electrophoresis (CE) allows a robust separation of chitosan by the degree of acetylation. The distribution of degrees of acetylation of various chitosan samples was characterized through their distributions of electrophoretic mobilities. These distributions can be obtained easily and with high precision. The heterogeneity of the chitosan chains in terms of acetylation was characterized through the dispersity of the electrophoretic mobility distributions obtained. The relationship between the number-average degree of acetylation obtained by solid-state NMR spectroscopy and the weight-average electrophoretic mobilities was established. The distribution of degrees of acetylation was determined using capillary electrophoresis in the critical conditions (CE-CC).

Keywords: Capillary electrophoresis; Chitosan; Distribution of degrees of acetylation.

MeSH terms

Absorption, Physicochemical
Acetylation
Chitosan / chemistry*
Phosphates / chemistry
Solubility
Surface Properties

Substances

Phosphates
Chitosan
sodium phosphate