A direct detection of human papillomavirus 16 genomic DNA using gold nanoprobes

Nor Azizah; Uda Hashim; Subash C B Gopinath; Sharipah Nadzirah

doi:10.1016/j.ijbiomac.2016.10.060

A direct detection of human papillomavirus 16 genomic DNA using gold nanoprobes

Int J Biol Macromol. 2017 Jan;94(Pt A):571-575. doi: 10.1016/j.ijbiomac.2016.10.060. Epub 2016 Oct 19.

Authors

Nor Azizah¹, Uda Hashim², Subash C B Gopinath³, Sharipah Nadzirah⁴

Affiliations

¹ Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia. Electronic address: norazizahparmin84@gmail.com.
² Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia. Electronic address: uda@unimap.edu.my.
³ Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia; School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia.
⁴ Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.

PMID: 27771413
DOI: 10.1016/j.ijbiomac.2016.10.060

Abstract

Nanoparticles have been investigated as flagging tests for the sensitive DNA recognition that can be utilized as a part of field applications to defeat restrictions. Gold nanoparticles (AuNPs) have been widely utilized due to its optical property and capacity to get functionalized with a mixed bag of biomolecules. This study exhibits the utilization of AuNPs functionalized with single-stranded oligonucleotide (AuNP-oligo test) for fast the identification of Human Papillomavirus (HPV). This test is displayed on interdigitated electrode sensor and supported by colorimetric assay. DNA conjugated AuNP has optical property that can be controlled for the applications in diagnostics. With its identification abilities, this methodology incorporates minimal effort, strong reagents and basic identification of HPV.

Keywords: Colorimetric detection; DNA detection; Gold nanoparticles; Human Papillomavirus; Interdigitated electrode sensor.

MeSH terms

Base Pairing
Colorimetry / methods*
DNA, Single-Stranded / chemical synthesis
DNA, Single-Stranded / chemistry
DNA, Viral / blood*
Electrodes
Gold / chemistry*
Human papillomavirus 16 / isolation & purification*
Humans
Metal Nanoparticles / chemistry*
Oligonucleotides / chemical synthesis
Oligonucleotides / chemistry
Sensitivity and Specificity
Silicon Dioxide / chemistry

Substances

DNA, Single-Stranded
DNA, Viral
Oligonucleotides
Gold
Silicon Dioxide