Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization

Nenad B Milosavić; Radivoje M Prodanović; Dušan Velićković; Aleksandra Dimitrijević

doi:10.1007/978-1-4939-6499-4_11

Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization

Methods Mol Biol. 2017:1504:139-147. doi: 10.1007/978-1-4939-6499-4_11.

Authors

Nenad B Milosavić¹, Radivoje M Prodanović², Dušan Velićković², Aleksandra Dimitrijević²

Affiliations

¹ Division of Experimental Therapeutics, Department of Medicine, Columbia University, 630 West 168th Street, P&S 8-436, New York, NY, 10032, USA. nm2729@columbia.edu.
² Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia.

PMID: 27770419
DOI: 10.1007/978-1-4939-6499-4_11

Abstract

One of the most used procedures for enzyme stabilization is immobilization. Although immobilization on solid supports has been pursued since the 1950s, there are no general rules for selecting the best support for a giving application. A macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate (poly (GMA-co-EGDMA)) is a carrier consisting of macroporous beads for immobilizing enzymes of industrial potential for the production of fine chemicals and pharmaceuticals.

Keywords: Covalent immobilization; Enzyme stabilization; Glutaraldehyde; Macroporous; Periodate; Poly (GMA-co-EGDMA).

MeSH terms

Aspergillus niger / chemistry
Aspergillus niger / enzymology*
Aspergillus niger / metabolism
Cross-Linking Reagents / chemistry*
Enzyme Stability
Enzymes, Immobilized / chemistry*
Enzymes, Immobilized / metabolism
Ethylene Glycols / chemistry*
Ethylene Oxide / chemistry
Glucan 1,4-alpha-Glucosidase / chemistry*
Glucan 1,4-alpha-Glucosidase / metabolism
Glutaral / chemistry*
Methacrylates / chemistry*
Oxidation-Reduction
Porosity

Substances

Cross-Linking Reagents
Enzymes, Immobilized
Ethylene Glycols
Methacrylates
poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)
Glucan 1,4-alpha-Glucosidase
Ethylene Oxide
Glutaral