Electrochemical sensor based on graphene oxide and ionic liquid for ofloxacin determination at nanomolar levels

Ademar Wong; Tiago Almeida Silva; Fernando Campanhã Vicentini; Orlando Fatibello-Filho

doi:10.1016/j.talanta.2016.08.035

Electrochemical sensor based on graphene oxide and ionic liquid for ofloxacin determination at nanomolar levels

Talanta. 2016 Dec 1:161:333-341. doi: 10.1016/j.talanta.2016.08.035. Epub 2016 Aug 15.

Authors

Ademar Wong¹, Tiago Almeida Silva¹, Fernando Campanhã Vicentini², Orlando Fatibello-Filho³

Affiliations

¹ Department of Chemistry, Federal University of São Carlos, 13560-970 São Carlos, SP, Brazil.
² Center of Nature Sciences, Federal University of São Carlos, 18290-000 Buri, SP, Brazil.
³ Department of Chemistry, Federal University of São Carlos, 13560-970 São Carlos, SP, Brazil. Electronic address: bello@ufscar.br.

PMID: 27769415
DOI: 10.1016/j.talanta.2016.08.035

Abstract

New insights into the design of highly sensitive, carbon-based electrochemical sensors are presented in this work by exploring the interesting properties of graphene oxide (GO) and ionic liquids (ILs). An electrochemical sensor based on the carbon paste electrode (CPE) modified with GO and IL was developed for the sensitive detection of ofloxacin using square-wave adsorptive anodic stripping voltammetry (SWAdASV). GO sheets were obtained from the acid treatment of graphene and characterized by scanning and transmission electronic microscopy (SEM and TEM) and selected area electron diffraction (SAED), and the electrochemical behavior of the modified GO-IL/CPE was explored by electrochemical impedance spectroscopy studies. The CPE modification with GO and IL allowed an 8.2 fold increase in the analytical sensitivity for ofloxacin sensing compared to the unmodified CPE. Under the optimized experimental conditions using the SWAdASV technique, the GO-IL/CPE sensor provided an analytical curve for ofloxacin in the concentration range of 7.0×10^-9 to 7.0×10^-7molL^-1, with a sensitivity of 7.7×10⁶μALmol^-1 and limit of detection of 2.8×10^-10molL^-1 (0.28nmolL^-1). The proposed sensor was successfully applied for the ofloxacin determination in human urine and ophthalmic samples, with recoveries near 100%. The results were similar those obtained by a spectrophotometric comparative method.

Keywords: Carbon paste electrode; Graphene oxide; Ionic liquid; Nanomolar concentration; Ofloxacin.