A New Dioic Acid from a wbl Gene Mutant of Deepsea-Derived Streptomyces somaliensis SCSIO ZH66

Mar Drugs. 2016 Oct 17;14(10):184. doi: 10.3390/md14100184.

Abstract

The wblAso gene functions as a global regulatory gene in a negative manner in deepsea-derived Streptomyces somaliensis SCSIO ZH66. A new dioic acid (1) as well as two known butenolides (2 and 3) were isolated from the ΔwblAso mutant strain of S. somaliensis SCSIO ZH66. The structure of 1 was elucidated by a combination of spectroscopic analyses, including MS and NMR techniques. In the cell growth inhibitory evaluation, compound 3 exhibited moderate activity against the human hepatic carcinoma cell line (Huh7.5) with an IC50 value of 19.4 μg/mL, while compounds 1 and 2 showed null activity up to 100 μg/mL.

Keywords: deepsea-derived Streptomyces; dioic acid; wblAso.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Bacteria / drug effects
  • Cell Line, Tumor
  • Chromatography, High Pressure Liquid
  • Dicarboxylic Acids / chemistry*
  • Dicarboxylic Acids / pharmacology*
  • Drug Screening Assays, Antitumor
  • Fermentation
  • Humans
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Microbial Sensitivity Tests
  • Mutation / genetics
  • Seawater / microbiology*
  • Streptomyces / chemistry
  • Streptomyces / genetics*
  • Streptomyces / metabolism*

Substances

  • Anti-Bacterial Agents
  • Antineoplastic Agents
  • Dicarboxylic Acids