Abstract
Histone deacetylases (HDACs) catalyze the deacetylation of lysine residues in their target proteins. This biochemical modification can have profound effects on the functions of these proteins and a dysregulation of HDAC activity contributes to severe diseases, including neoplastic transformation. In the following chapter, we present a strategy that allows to distinguish between the inhibition of the class I HDACs HDAC1, 2, and 3 and of the class IIb HDAC HDAC6. This method is based on Western blot and relies on the detection of hyperacetylated substrates of class I or class IIb HDACs in lysates from cells that were treated with histone deacetylase inhibitors (HDACi).
Keywords:
Acetylation; HDAC6; HDACi; HDACs; Histone; Tubulin.
MeSH terms
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Acetylation
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Animals
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Benzamides / pharmacology
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Blotting, Western / methods
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Cell Line, Tumor
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Epigenesis, Genetic
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Epithelial Cells / cytology
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Epithelial Cells / drug effects
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Epithelial Cells / enzymology
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Histone Deacetylase 1 / antagonists & inhibitors
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Histone Deacetylase 1 / genetics
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Histone Deacetylase 1 / metabolism*
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Histone Deacetylase 2 / antagonists & inhibitors
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Histone Deacetylase 2 / genetics
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Histone Deacetylase 2 / metabolism*
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Histone Deacetylase 6 / antagonists & inhibitors
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Histone Deacetylase 6 / genetics
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Histone Deacetylase 6 / metabolism*
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Histone Deacetylase Inhibitors / pharmacology*
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Histone Deacetylases / genetics
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Histone Deacetylases / metabolism*
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Histones / genetics
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Histones / metabolism
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Humans
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Hydroxamic Acids / pharmacology
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Indoles / pharmacology
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Mice
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Monocytes / cytology
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Monocytes / drug effects
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Monocytes / enzymology
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Panobinostat
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Protein Processing, Post-Translational*
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Pyridines / pharmacology
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Valproic Acid / pharmacology
Substances
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Benzamides
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Histone Deacetylase Inhibitors
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Histones
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Hydroxamic Acids
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Indoles
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Pyridines
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entinostat
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Valproic Acid
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Panobinostat
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HDAC1 protein, human
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HDAC2 protein, human
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HDAC6 protein, human
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Histone Deacetylase 1
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Histone Deacetylase 2
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Histone Deacetylase 6
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Histone Deacetylases
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histone deacetylase 3