Abstract
Here we describe the method used in our laboratory for determining the activity of homologous recombination repair of DNA double-strand breaks in cell lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin's laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.
Keywords:
Class I histone deacetylases; Homologous recombination; Valproic acid.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Line, Tumor
-
DNA / genetics
-
DNA / metabolism
-
DNA Breaks, Double-Stranded*
-
Flow Cytometry / methods
-
Green Fluorescent Proteins / antagonists & inhibitors
-
Green Fluorescent Proteins / genetics*
-
Green Fluorescent Proteins / metabolism
-
Histone Deacetylase 1 / genetics*
-
Histone Deacetylase 1 / metabolism
-
Histone Deacetylase Inhibitors / pharmacology*
-
Humans
-
Isoenzymes / antagonists & inhibitors
-
Isoenzymes / genetics
-
Isoenzymes / metabolism
-
Melanocytes / cytology
-
Melanocytes / drug effects
-
Melanocytes / metabolism
-
Plasmids / chemistry
-
Plasmids / metabolism
-
Puromycin / pharmacology
-
Recombinant Fusion Proteins
-
Recombinational DNA Repair / drug effects*
-
Valproic Acid / pharmacology
Substances
-
Histone Deacetylase Inhibitors
-
Isoenzymes
-
Recombinant Fusion Proteins
-
Green Fluorescent Proteins
-
Puromycin
-
Valproic Acid
-
DNA
-
HDAC1 protein, human
-
Histone Deacetylase 1