Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination

Methods Mol Biol. 2017:1510:115-123. doi: 10.1007/978-1-4939-6527-4_9.

Abstract

Here we describe the method used in our laboratory for determining the activity of homologous recombination repair of DNA double-strand breaks in cell lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin's laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.

Keywords: Class I histone deacetylases; Homologous recombination; Valproic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • DNA / genetics
  • DNA / metabolism
  • DNA Breaks, Double-Stranded*
  • Flow Cytometry / methods
  • Green Fluorescent Proteins / antagonists & inhibitors
  • Green Fluorescent Proteins / genetics*
  • Green Fluorescent Proteins / metabolism
  • Histone Deacetylase 1 / genetics*
  • Histone Deacetylase 1 / metabolism
  • Histone Deacetylase Inhibitors / pharmacology*
  • Humans
  • Isoenzymes / antagonists & inhibitors
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Melanocytes / cytology
  • Melanocytes / drug effects
  • Melanocytes / metabolism
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Puromycin / pharmacology
  • Recombinant Fusion Proteins
  • Recombinational DNA Repair / drug effects*
  • Valproic Acid / pharmacology

Substances

  • Histone Deacetylase Inhibitors
  • Isoenzymes
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Puromycin
  • Valproic Acid
  • DNA
  • HDAC1 protein, human
  • Histone Deacetylase 1