Abstract
β-Conglycinin (β-CG) is well known for inducing intestinal allergies and dysfunction in neonates and young pigs. However, the underlying mechanisms are largely unknown. In this study, to clarify the role of autophagy in β-CG-induced cytotoxicity, we investigated the effects of β-CG on cell viability and autophagy activity in porcine enterocytes (IPEC-1 cells). The results indicated that the cell viability was decreased with the increasing levels of β-CG. β-CG treatment enhanced the eGFP-LC3 puncta per cells and LC3-II/LC3-I, and the latter was further increased in IPEC-1 cells cultured with bafilomycin A1. We conclude that β-CG enhances autophagy activity in enterocytes.
Keywords:
Autophagy; IPEC-1 cells; Microtuble-associated protein light chain 3; β-Conglycinin.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Allergens / isolation & purification
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Allergens / pharmacology*
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Animal Feed / analysis
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Animals
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Antigens, Plant / isolation & purification
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Antigens, Plant / pharmacology*
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Autophagy / drug effects*
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Autophagy / genetics
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Cell Line
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Cell Survival / drug effects
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Enterocytes
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Gene Expression Regulation
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Globulins / isolation & purification
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Globulins / pharmacology*
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Glycine max / chemistry*
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Macrolides / pharmacology
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Seed Storage Proteins / isolation & purification
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Seed Storage Proteins / pharmacology*
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Soybean Proteins / isolation & purification
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Soybean Proteins / pharmacology*
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Swine
Substances
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Allergens
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Antigens, Plant
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Globulins
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Macrolides
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Microtubule-Associated Proteins
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Seed Storage Proteins
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Soybean Proteins
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beta-conglycinin protein, Glycine max
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bafilomycin A