Noninvasive Tracking of Alginate-Microencapsulated Cells

Genaro A Paredes-Juarez; Brad P Barnett; Jeff W M Bulte

doi:10.1007/978-1-4939-6364-5_11

Noninvasive Tracking of Alginate-Microencapsulated Cells

Methods Mol Biol. 2017:1479:143-155. doi: 10.1007/978-1-4939-6364-5_11.

Authors

Genaro A Paredes-Juarez^{1

2}, Brad P Barnett³, Jeff W M Bulte^{4

5

6

7

8}

Affiliations

¹ Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
² Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave., Baltimore, MD, 21205, USA.
³ Wilmer Ophthalmological Institute, Johns Hopkins Hospital, Baltimore, MD, 21287-9115, USA.
⁴ Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.
⁵ Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave., Baltimore, MD, 21205, USA. jwmbulte@mri.jhu.edu.
⁶ Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.
⁷ Department of Chemical & Biomolecular Engineering, The Johns Hopkins Whiting School of Engineering, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.
⁸ Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.

Abstract

This chapter presents a description of standardized techniques used routinely in our laboratory to encapsulate different cell types using the alginate-PLL-alginate immunoisolation system. Given the importance of noninvasive tracking of encapsulated cell transplants, we present a detailed guidance to achieve maximum efficiency and functionality of the capsule preparations for optimal tracking posttransplantation. The provided protocols cover tracking of encapsulated cells using magnetic resonance (MR), X-ray, computed tomography (CT), and ultrasound (US) imaging. Practical suggestions to optimize each method with specific references to recommended suppliers are included.

Keywords: Alginate; Encapsulation; MRI; Noninvasive tracking; Ultrasound; X-ray.

MeSH terms

Alginates / chemistry*
Animals
Capsules / chemistry
Cell Tracking / methods*
Cells, Immobilized / cytology*
Drug Compounding / instrumentation
Drug Compounding / methods
Equipment Design
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Humans
Immunosuppression Therapy
Magnetic Resonance Imaging / methods
Tomography, X-Ray Computed / methods
Ultrasonography / methods

Substances

Alginates
Capsules
Hexuronic Acids
Glucuronic Acid

Abstract

MeSH terms

Substances

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