Updated Multiplex PCR for Detection of All Six Plasmid-Mediated qnr Gene Families

Antimicrob Agents Chemother. 2016 Nov 21;60(12):7524-7526. doi: 10.1128/AAC.01447-16. Print 2016 Dec.

Abstract

Plasmid-mediated qnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance in multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR in order to detect all six qnr families (including qnrA, qnrS, qnrB, qnrC, qnrD, and qnrVC) described until now. The proposed method makes possible the screening of these genes, reducing cost and time, and it may demonstrate an underestimated prevalence of the latest variants described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification*
  • Bacterial Proteins / metabolism
  • DNA Primers / chemical synthesis
  • DNA Primers / metabolism
  • Databases, Genetic
  • Drug Resistance, Bacterial / genetics*
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / genetics*
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae / metabolism
  • Enterobacteriaceae Infections / microbiology
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / isolation & purification*
  • Escherichia coli Proteins / metabolism
  • Gene Expression
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Plasmids / chemistry
  • Plasmids / metabolism*
  • Protein Isoforms / genetics
  • Protein Isoforms / isolation & purification
  • Protein Isoforms / metabolism
  • Quinolones / pharmacology

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA Primers
  • Escherichia coli Proteins
  • Protein Isoforms
  • Qnr protein, E coli
  • Qnr protein, Klebsiella pneumoniae
  • Quinolones