Abstract
We studied the significance of particular eis mutations on Mycobacterium tuberculosis drug resistance using a specialized transduction strategy. Recombinant strains harboring eis promoter mutations C14T, C12T, and G10A exhibited kanamycin resistance with MICs of 40, 10, and 20 μg/ml, respectively, while recombinant strains harboring C14G and C15G mutations were kanamycin susceptible (MIC, 2.5 to 5 μg/ml). Each of the eis mutants tested remained amikacin susceptible (MIC, 0.5 to 4 μg/ml). The identification of specific eis mutations is needed for accurate genotypic susceptibility testing for kanamycin.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Acetyltransferases
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Amikacin / pharmacology
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Antigens, Bacterial / genetics*
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Antigens, Bacterial / metabolism
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Antitubercular Agents / pharmacology*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Gene Expression
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Genotype
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Kanamycin / pharmacology*
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Kanamycin Resistance / genetics
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Mutation*
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / growth & development
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Promoter Regions, Genetic
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Transduction, Genetic
Substances
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Antigens, Bacterial
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Antitubercular Agents
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Bacterial Proteins
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Kanamycin
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Amikacin
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Acetyltransferases
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Eis protein, Mycobacterium tuberculosis