Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.
Keywords: Gel exclusion; Gel permeation; Gel-filtration chromatography; Molecular mass estimation; Molecular sieve; Operating conditions; Separations; Size exclusion; Size-exclusion reaction chromatography.