Sensitivity of photosynthetic processes to freezing temperature in extremophilic lichens evaluated by linear cooling and chlorophyll fluorescence

Abstract

Extremophilic lichens and their photosynthesizing photobionts from the cold regions of Earth are adapted to perform photosynthesis at subzero temperatures. To evaluate interspecific differences in the critical temperature for primary photochemical processes of photosynthesis, we exposed lichen thalli of Usnea antarctica, Usnea aurantiaco-atra, and Umbilicaria cylindrica to linear cooling from +20 to -50 °C at a constant rate of 2 °C min^-1. Simultaneously, two chlorophyll fluorescence parameters (F_V/F_M - potential yield of photosynthetic processes in photosystem II, Φ_PSII - effective quantum yield of PS II) evaluating a gradual subzero temperature-induced decline in photosynthetic processes were measured by a modulated fluorometer. For the studied species, the response of F_V/F_M and Φ_PSII to declining temperature showed an S-curve shape. The decline in F_V/F_M and Φ_PSII at low temperatures started at -5 and +5 °C, respectively in the majority of cases. The decline was, however, species-specific. U. aurantiaco-atra showed a constant-rate decline of Φ_PSII from the physiological temperature 20 °C. U. antarctica exhibited the first sign of F_V/F_M decline at -12 °C. The critical temperature related to full inhibition of the photosynthetic processes in PSII (F_V/F_M), was found at -20 °C. However, this occurred at -30 °C for U. cylindrica. In an individual sample, the critical temperature for F_V/F_M was typically lower than for Φ_PSII. The method of linear cooling combined with simultaneous measurements of chlorophyll fluorescence parameters proved to be an efficient tool in the estimation of extremophilic species sensitivity/resistance to freezing.

Keywords: Antarctica; Cryoresistance; James ross island; Temperature stress; Umbilicaria cylindrica; Usnea antarctica; Usnea aurantiaco-atra.