Abstract
Two new phenylspirodrimane derivatives, stachybotrysin (1) and stachybotrylactone B (2), were isolated from the cultures of the marine-derived fungus Stachybotrys sp. KCB13F013. The structures were determined by analyzing the spectroscopic data (1D and 2D NMR and MS) and chemical transformation, including the modified Mosher's method and single-crystal X-ray structure analysis. Compound 1 exhibited an inhibitory effect on osteoclast differentiation in bone marrow macrophage cells via suppressing the RANKL-induced activation of p-ERK, p-JNK, p-p38, c-Fos, and NFATc1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow Cells / drug effects
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Cell Differentiation / drug effects
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Coculture Techniques
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Down-Regulation / drug effects
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Macrophages / drug effects
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Marine Biology
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Mice
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Molecular Structure
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NF-kappa B / antagonists & inhibitors
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Osteoblasts / drug effects
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Osteoclasts / drug effects*
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Proto-Oncogene Proteins c-fos / genetics
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RANK Ligand / pharmacology
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Signal Transduction / drug effects
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Stachybotrys / chemistry*
Substances
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NF-kappa B
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Proto-Oncogene Proteins c-fos
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RANK Ligand
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TNFSF11 protein, human