Abstract
We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings.
MeSH terms
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Chlamydia trachomatis / genetics
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Chlamydia trachomatis / isolation & purification
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DNA, Bacterial / analysis*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Electrophoresis, Capillary
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Humans
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Magnetics
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Molecular Diagnostic Techniques / methods*
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Neisseria gonorrhoeae / genetics
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Neisseria gonorrhoeae / isolation & purification
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Nucleic Acid Amplification Techniques / methods*
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Sexually Transmitted Diseases / diagnosis*
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Sexually Transmitted Diseases / microbiology*